delta-Aminolevulinate synthase isozymes in the liver and erythroid cells of chicken |
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Authors: | N Watanabe N Hayashi G Kikuchi |
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Institution: | Department of Biochemistry, Tohoku University School of Medicine, Sendai 980, Japan |
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Abstract: | Antibodies raised against the purified chicken liver delta-aminolevulinate synthase showed a partial cross-reactivity with the chicken erythroid delta-aminolevulinate synthase. delta-Aminolevulinate synthase synthesized in vitro using polysomes from erythroid cells showed a subunit molecular weight of 55,000, whereas the enzyme synthesized in vitro using liver polysomes had a subunit molecular weight of 73,000. delta-Aminolevulinate synthase isolated from mitochondria of erythroid cells showed a molecular weight of 53,000, while the enzyme in liver mitochondria had a value of 65,000. These observations imply that the erythroid delta-aminolevulinate synthase differs from the hepatic enzyme. |
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Keywords: | AIA allylisopropylacetamide DDC 3 5-dicarbethoxy-1 4-dihydrocollidine SDS sodium dodecyl sulfate |
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