红曲菌利用生物柴油废液生产红曲色素

对生物柴油废液作简单处理，利用红曲茵发酵生物柴油废液中副产物甘油生产红曲色素。通过响应面方法确定最佳发酵培养基为：甘油48．49g/L，蛋白胨3．12g／L，K2HPO4·3H202．01g/L，MgSO4 0．48g／L，ZnSO4·7H2O 0．04g／L，MnSO4·H2O 0．03g／L，玉米浆13mL／L，植物油10mL／L，起始pH为6。发酵结果表明：在接种量6％（v／v），转速140r／min，35℃的条件下发酵培养6d，红曲色素最高产量到达204U／mL。说明用生物柴油废液中的粗甘油为原料生产红曲色素是基本可行的。可望为生物柴油废液的资源化提供一条环境友好型的途径。

Monascus pigment production from glycerol-containing wastes discharged after biodiesel manufacturing process

After simple pre-treatment, the glycerol-containing waste discharged after biodiesel manufacturing process was used for production of Monascus pigments by Monascus sp. 9903A. The fermentation medium optimized by using response surface methodology were as follows： crude glycerol 48.49g/L, peptone 3.12g/L, K2 HPO4 3H2O 2.01g/L, MgSO4 0.48g/L, ZnSO4 ·7H2O 0.04g/L, MnSO4. H2O 0.03g/L, corn steep liquor 13mL/L, vegetable oil 10mL/L, initial pH6. The batch fermentation in shake flasks was conducted at 35℃ for 6 days, with inoculum 6 % （v/v）, shaking at 140r/min. The pigment production yield of Monascus 9903A reached 204U/mL, indicating that it was feasible to use the glycerol-containing waste after biodiesel manufacturing process for manufacturing Monascus pigments. The process offered an environmental friendly approach to the recycle of biodiesel wastes.