杧果LEAFY同源基因的分离及序列分析

分析在植物开花过程中起重要作用的LEAFY(LFY)基因的保守区序列,设计1对长度均为23bp的PCR引物,以杧果基因组DNA为模板,采用PCR方法扩增出长为822bp的DNA片段,克隆入pGEM-T Easy载体。测序和序列分析表明,获得了杧果LFY同源基因(miLFY)3’端的1个片段,该片段有1个415bp的内含子,编码区共编码135个氨基酸,其序列已经在GenBank中登记(登录号AY189684)。在GenBank中进行同源性检索,发现其氨基酸序列与其它植物LFY同源基因的氨基酸序列同源性高达74%~97%,推测它们具有相似的功能。

Isolation and sequence analysis of LEAFY homologous gene from mango

Analyzing a conserved sequence of the LEAFY (LFY) gene, which plays an important role in floral development, a pair of 23 bp primers was used to amplify an 822 bp fragment by polymerase chain (PCR) with genomic DNA of mango. The fragment was cloned into pGEM-T Easy vector, and then sequenced. A fragment in the 3'-end of LFY homologous gene named miLFY gene was obtained. The result of sequence analysis indicated that there was an intron of 415 bp in the fragment, and the exons encoded 135 amino acids. The miLFY gene has been registered in GenBank with the accession number AY189684. After the deduced amino acid sequence of the fragment was submitted to GenBank and blast with LFY homologous gene of other plants, it was found that the homology reached 74%~97%. This result suggested that miLFY gene might have the same function as other LFY homologous gene.