| 血管紧张素Ⅱ对缺血心肌细胞钾离子通道的作用 |
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| 引用本文： | Wang WW,Zhu YC,Yao T,Zheng P,Gong QL. 血管紧张素Ⅱ对缺血心肌细胞钾离子通道的作用[J]. 生理学报, 2002, 54(2): 149-153 |
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| 作者姓名： | Wang WW Zhu YC Yao T Zheng P Gong QL |
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| 作者单位： | 复旦大学基础医学院生理学教研室,国家医学神经生物学重点实验室,上海,200032 |
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| 基金项目： | ThisworkwassupportedbyShanghaiShuguangProject (No 97SG15 ) |
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| 摘 要： | 实验用胶原酶酶解法急性分离豚鼠心室肌细胞,利用全细胞膜片钳的方法记录心室肌细胞的延迟整流钾电流(Ik）、内向整流钾电流（Ik1）和ATP敏感钾电流（IKATP）。采用低氧、无糖、高乳酸和酸中毒综合方式模拟缺血灌流,造成细胞的模拟缺血,并在缺血的基础上继续用含100nmol/L AngⅡ灌流细胞,观察Ang Ⅱ对模拟缺血心室肌细胞钾离子通道的影响。实验结果显示：（1）模拟缺血时,Ik明显减小;Ang Ⅱ能进一步抑制Ik。（2）模拟缺血条件下,Ik1受到抑制,并且以内向电流的抑制为主;Ang Ⅱ可加强对Ik1内向电流的抑制,而对部分外向电流则有增加的作用。（3）模拟缺血使IKATP外向电流略有增加;Ang Ⅱ则明显加强IKATP外向电流,此效应能被优降糖所阻断。
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| 关 键 词： | 全细胞膜片钳 心肌缺血 血管紧张素Ⅱ 心肌细胞 钾离子通道 |
| 修稿时间： | 2001-07-15 |
Effect of angiotensin II on potassium channels of ischemic ventricular myocytes of the guinea pig |
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| Wang Wen-Wei,Zhu Yi-Chun,Yao Tai,Zheng Ping,Gong Qian-Ling. Effect of angiotensin II on potassium channels of ischemic ventricular myocytes of the guinea pig[J]. Acta Physiologica Sinica, 2002, 54(2): 149-153 |
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| Authors: | Wang Wen-Wei Zhu Yi-Chun Yao Tai Zheng Ping Gong Qian-Ling |
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| Affiliation: | Department of Physiology, Medical Center of Fudan University, Shanghai 200032. wangwenwei66@yahoo.com |
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| Abstract: | The experiments were carried out on guinea pig isolated ventricular myocytes by using whole-cell patch clamp. The effects of angiotensin II (Ang II) on potassium ion channels of acute ischemic myocytes were observed. Whole-cell patch clamp recordings showed that physiological potassium current, including delayed rectifier potassium current and inward rectifier potassium current were inhibited under the condition of simulated ischemia, and then further inhibited by treatment with Ang II. ATP-sensitive potassium currents were increased under simulated ischemia and were further enhanced by Ang II treatment. |
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