The use of ROI overlays and a semi-automated method for measuring cortical area in ImageJ for histological analysis |
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Authors: | Victoria M Dominguez Amanda M Agnew |
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Institution: | Skeletal Biology Research Laboratory, The Ohio State University, Columbus, Ohio |
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Abstract: | A major part of histologic studies is the use of high resolution imaging for data collection and analysis. ImageJ, a freely available software from the NIH designed for image analysis, has many features that are not well-known among bone histologists and can be incredibly beneficial in terms of stream-lining data collection and maximizing limited resources. The aims of this technical note are twofold: (a) to describe methods for image annotation and measurement using region of interest overlays in ImageJ, and (b) to present a new code for a semi-automated method of measuring cortical bone areas from high resolution cross-sectional images also using ImageJ. |
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Keywords: | bone histology cortical area histomorphometry ImageJ |
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