Combined profile of androgen glucuro- and sulfoconjugates in post-competition urine of sportsmen: a simple screening procedure using gas chromatography-mass spectrometry |
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| Institution: | 1. Department of Analytic Human Pathology, Graduate School of Medicine, Nippon Medical School, Tokyo 113-0031, Japan;2. Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, Nippon Medical School, Tokyo 113-8602, Japan;3. Nippon Medical School, Chiba Hokusoh Hospital, Respiratory Disease Center, Chiba 270-1694, Japan;4. Department of Pathology, Nippon Medical School, Chiba Hokusoh Hospital, Chiba 270-1694, Japan;1. Department of Growth and Reproduction, EDMaRC, Rigshospitalet, Denmark;2. Faculty of Health and Medical Sciences, University of Copenhagen, Denmark;1. Department of Gastroenterology of Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou, 570108, China;2. Department of Oncology Rehabilitation and Palliative Care, The First Affiliated Hospital of Hainan Medical University, Haikou 570108, China;3. Center Laboratory of Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou 570108, China;1. Faculty of Life and Environmental Sciences, University of Tsukuba, Japan;2. Tateyama Marine Biological Station, Ochanomizu University, Japan;1. Department of Endocrinology and Metabolism, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China;2. Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China;3. The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Heilongjiang Province, Harbin 150086, China |
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| Abstract: | An analytical screening procedure has been developed for the estimation of total androgen conjugates in post-competition urine, using gas chromatography-mass spectrometry with computerized data acquisition and concentration calculation. Rapid acid-catalyzed methanolysis is a key feature of the method, which allows simulatenous cleavage of glucuronides and sulfates. Analytical data generated by this method for testosterone and epitestosterone are in accordance with our previous results obtained by more accurate isotope dilution mass spectrometry. The usefulness of the ratio of testosterone glucuronide-total epitestosterone as an aid for a better discrimination between physiologically high and pharmacologically high ratios of testosterone glucuronide-epitestosterone glucuronide, which was demonstrated previously, has been confirmed here. |
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