Biosynthesis of epsilon-poly-L-lysine in a cell-free system of Streptomyces albulus |
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Authors: | Kawai Takahiro Kubota Takaaki Hiraki Jun Izumi Yoshikazu |
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Affiliation: | Department of Biotechnology, Faculty of Engineering, Tottori University, Koyama-minami, Tottori 680-8552, Japan. |
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Abstract: | epsilon-Poly-L-lysine (epsilon-PL) is a homo-poly-amino acid characterized by a peptide bond between carboxyl and epsilon-amino groups of L-lysine. Here we report the cell-free synthesis of epsilon-PL by a sensitive radioisotopic epsilon-PL assay system. In vitro epsilon-PL synthesis depended on ATP and was not affected by ribonuclease, kanamycin, or chloramphenicol. epsilon-PL synthesizing activity was detected in the membrane fraction. The reaction product, epsilon-PL, from L-lysine was identified by MALDI-TOF MS and the number of lysine residues of the epsilon-PL products was apparently 11-34. These results suggest that the biosynthesis of epsilon-PL is nonribosomal peptide synthesis and is catalyzed by membrane bound enzyme(s). The enzyme preparation showing the epsilon-PL synthesizing activity also catalyzed lysine-dependent AMP production and an ATP-PPi exchange reaction, suggesting that L-lysine is adenylated in the first step of epsilon-PL biosynthesis. |
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Keywords: | ε-Poly- smallcaps" >l-lysine Homo-poly-amino acid Nonribosomal peptide synthesis ATP-PPi exchange reaction |
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