Expression pattern of enzymes related to juvenile hormone metabolism in the silkworm, <Emphasis Type="Italic">Bombyx mori</Emphasis> L. |
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Authors: | Yang Hua-jun Zhou Fang Sabhat Awquib Firdose Ahmad Malik Bhaskar Roy Li Xing-hua Hu Jia-biao Sun Chun-guang Yan-shan Niu Miao Yun-gen |
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Institution: | (1) Key Laboratory of Animal Epidemic Etiology & Immunological Prevention of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310029, People’s Republic of China |
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Abstract: | The physiological balance of juvenile hormone (JH) in insects depends on its biosynthesis and degradation pathway. Three key
enzymes namely, juvenile hormone esterase (JHE), juvenile hormone epoxide hydrolase (JHEH) and juvenile hormone diol kinase
(JHDK) are required for degradation in insects. Our present results showed that JHE and JHEH exhibited expression in almost
all the tissues. This indicated that JHE and JHEH might degrade JH simultaneously. In addition, the highest levels of JHDK
were observed in the midgut, with trace level being found in the malpighian tubule and haemocytes. Since the midgut is a digestive
organ and not a JH target, it was hypothesized that both JHE and JHEH hydrolyzed JH to JH diol (JHd) which was then transported
to midgut and hydrolyzed further by JHDK, to be finally excreted out of the body. Also the expression studies on JH degradation
enzymes in different tissues and stages indicated that the activities of the three enzymes are specific and coincident with
the JH functions in silkworm, Bombyx mori L. |
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