Leishmania major chromosome 3 contains two long convergent polycistronic gene clusters separated by a tRNA gene |
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Authors: | Worthey E A Martinez-Calvillo Santiago Schnaufer Achim Aggarwal Gautam Cawthra Jason Fazelinia Gholam Fong Chris Fu Guoliang Hassebrock Melissa Hixson Greg Ivens Alasdair C Kiser Patti Marsolini Felicia Rickel Erika Rickell Erica Salavati Reza Sisk Ellen Sunkin Susan M Stuart Kenneth D Myler Peter J |
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Affiliation: | Seattle Biomedical Research Institute, 4 Nickerson Street, Seattle, WA 98109-1651, USA. |
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Abstract: | Leishmania parasites (order Kinetoplastida, family Trypanosomatidae) cause a spectrum of human diseases ranging from asymptomatic to lethal. The ~33.6 Mb genome is distributed among 36 chromosome pairs that range in size from ~0.3 to 2.8 Mb. The complete nucleotide sequence of Leishmania major Friedlin chromosome 1 revealed 79 protein-coding genes organized into two divergent polycistronic gene clusters with the mRNAs transcribed towards the telomeres. We report here the complete nucleotide sequence of chromosome 3 (384 518 bp) and an analysis revealing 95 putative protein-coding ORFs. The ORFs are primarily organized into two large convergent polycistronic gene clusters (i.e. transcribed from the telomeres). In addition, a single gene at the left end is transcribed divergently towards the telomere, and a tRNA gene separates the two convergent gene clusters. Numerous genes have been identified, including those for metabolic enzymes, kinases, transporters, ribosomal proteins, spliceosome components, helicases, an RNA-binding protein and a DNA primase subunit. |
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