Modifying lysine biosynthesis and catabolism in corn with a single bifunctional expression/silencing transgene cassette |
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Authors: | Frizzi Alessandra Huang Shihshieh Gilbertson Larry A Armstrong Toni A Luethy Michael H Malvar Thomas M |
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Affiliation: | Mystic Research, Monsanto Company, 62 Maritime Drive, Mystic, CT 06355, USA; Chesterfield Campus, Monsanto Company, 700 Chesterfield Parkway West Chesterfield, MO 63017, USA |
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Abstract: | Although it is one of the major crops in the world, corn has poor nutritional quality for human and animal consumption due to its low lysine content. Here, we report a method of simultaneous expression of a deregulated lysine biosynthetic enzyme, CordapA, and reduction of a bifunctional lysine degradation enzyme, lysine-ketoglutarate reductase/saccharophine dehydrogenase (LKR/SDH), in transgenic corn plants by a single transgene cassette. This is accomplished by inserting an inverted-repeat sequence targeting the maize LKR/SDH gene into an intron of a transgene cassette that expresses CordapA. This combination of LKR/SDH silencing and CordapA expression led to the accumulation of free lysine to over 4000 p.p.m. in transgenic corn grain, compared to less than 100 p.p.m. in wild-type controls. This intron-embedded silencing cassette design reduces the number of transgene cassettes needed in transgenic approaches for manipulating metabolic pathways that sometimes require expression of one gene and silencing of another. |
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Keywords: | high lysine corn quality trait double-stranded RNA intron-embedded dihydrodipicolinate synthase (DHDPS) CordapA lysine-ketoglutarate reductase/saccharophine dehydrogenase (LKR/SDH) |
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