Effects of plant growth regulators and <Emphasis Type="SmallCaps">l</Emphasis>-glutamic acid on shoot organogenesis in the halophyte <Emphasis Type="Italic">Leymus chinensis</Emphasis> (Trin.) |
| |
Authors: | Yan-Lin Sun Soon-Kwan Hong |
| |
Institution: | (1) Department of Plant Biotechnology, Division of Biotechnology, College of Biotechnology, Kangwon National University, Chuncheon, Kangwon-Do, 200-701, Korea;(2) Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Kangwon-Do, 200-701, Korea |
| |
Abstract: | The halophyte Leymus chinensis (Trin.) is a perennial rhizome grass (tribe Gramineae) that is widely distributed in China, Mongolia and Siberia, where it
is produced as a forage product. In this report, we establish a highly reproducible plant regeneration system through somatic
embryogenesis. Two explants, mature seeds and leaf base segments were used; these parts displayed different responses to combinations
of growth factors that affect embryogenic callus induction, callus type optimization and plant regeneration. The highest callus
induction frequency was obtained on Murashige and Skoog (MS) medium supplemented with 2.0 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) in the presence of 5.0 mg l−1
l-glutamic acid. The inclusion of 5.0 mg l−1
l-glutamic acid was found to significantly promote primary callus induction, embryogenic callus formation and callus status
improvement. Subculturing on maintenance medium for 1–2 months before plant regeneration was found to be essential for the
optimization of callus type and the maturation of embryogenic callus. Callus relative water content and growth rate were simultaneously
investigated during callus maintenance, and found to possibly be related to callus type. Shoots were differentiated from the
embryogenic callus on the optimal medium with MS salts containing 0.2–0.5 mg l−1 α-naphthalene acetic acid (NAA), 2.0 mg l−1 kinetin (Kn) and 2.0 g l−1 casamino acids in 71.0 and 69.2% of wild-type (WT) and Jisheng No.1 (JS) plants, respectively. Plant regeneration was variable
depending on NAA levels, and the addition of casamino acids stimulated the maturation of embryogenic callus and plant regeneration.
Transferring callus with shoots onto half-strength MS medium resulted in rooting within 1 week. The growth of regenerated
plants was also surveyed in the field. This is the first report of plant regeneration through somatic embryogenesis from mature
seeds and leaf base segments of L. chinensis. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|