In vivo detection of c-Met expression in a rat C6 glioma model |
| |
Authors: | Towner R A Smith N Doblas S Tesiram Y Garteiser P Saunders D Cranford R Silasi-Mansat R Herlea O Ivanciu L Wu D Lupu F |
| |
Institution: | Small Animal MRI Core Facility, Oklahoma City, OK, USA. Rheal-Towner@omrf.org |
| |
Abstract: | The tyrosine kinase receptor, c-Met, and its substrate, the hepatocyte growth factor (HGF), are implicated in the malignant progression of glioblastomas. In vivo detection of c-Met expression may be helpful in the diagnosis of malignant tumours. The C6 rat glioma model is a widely used intracranial brain tumour model used to study gliomas experimentally. We used a magnetic resonance imaging (MRI) molecular targeting agent to specifically tag the cell surface receptor, c-Met, with an anti-c-Met antibody (Ab) linked to biotinylated Gd (gadolinium)-DTPA (diethylene triamine penta acetic acid)-albumin in rat gliomas to detect overexpression of this antigen in vivo. The anti-c-Met probe (anti-c-Met-Gd-DTPA-albumin) was administered intravenously, and as determined by an increase in MRI signal intensity and a corresponding decrease in regional T(1) relaxation values, this probe was found to detect increased expression of c-Met protein levels in C6 gliomas. In addition, specificity for the binding of the anti-c-Met contrast agent was determined by using fluorescence microscopic imaging of the biotinylated portion of the targeting agent within neoplastic and 'normal'brain tissues following in vivo administration of the anti-c-Met probe. Controls with no Ab or with a normal rat IgG attached to the contrast agent component indicated no non-specific binding to glioma tissue. This is the first successful visualization of in vivo overexpression of c-Met in gliomas. |
| |
Keywords: | molecular-targeted MRI c-Met gliomas biotinylated-anti-c-Met-Gd-DTPA-albumin rat in vivo |
本文献已被 PubMed 等数据库收录! |
|