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Phyto-extraction of heavy metals and biochemical changes with Brassica nigra L. grown in rayon grade paper mill effluent irrigated soil
Authors:Uday Veer Singh  Amar Abhishek  Monika Bhaskar  Neeraj Tandan  Nasreen Ghazi Ansari  Netra Pal Singh
Abstract:In this study, distribution of metal accumulation and their biological changes of Indian mustard plants (Brassica nigra L.) grown insoil irrigated with different concentration of rayon grade paper effluent (RGPE, 25%, 50%, 75%, 100%, v/v) were studied. Apronounced effect was recorded at 50% (v/v) RGPE on germination of seeds, amylase activity and other growth parameters inIndian mustard plants. An increase in the chlorophyll and protein contents was also recorded at <50% (v/v) RGPE followed by adecrease at higher concentrations of RGPE (>75%). A significant increase lipid peroxidation was recorded, which was evidenced bythe increased malondialdehyde (MDA) content in shoot, leaves and seeds in tested plant at all the concentrations of RGPE. ThisIndian mustard plants (Brassica nigra L.) are well adapted for tolerance of significant amount of heavy metals due to increased levelof antioxidants (cysteine and ascorbic acid) in root shoot and leaves of treated plants at all concentration of RGPE. Moreover, it isalso important that RGPE should be treated to bring down the metal concentration well within the prescribed limit prior to use inagricultural soil for ferti-irrigation.
Keywords:Rayon grade paper mill effluent   Indian mustard plant   Phytoremediation   Heavy metals   Lipid peroxidation   biochemical parameters
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