Quartz sand as an adsorbent for purification of extracellular glucose oxidase from Penicillium funiculosum 46.1

The procedure of purification of extracellular glucose oxidase (GO, EC 1.1.3.4) from culture-liquid filtrate (CLF) of the fungus Penicillum funiculosum 46.1 using alluvial quartz sand as an adsorbent has been developed. The modification of sand by changing the charge and polarity did not lead to a significant increase in its adsorption capacity towards GO. The effectiveness of sand and aluminum oxide, used as sorbents for isolation of GO from CLF, was compared. Glucose oxidase, isolated from CLF by adsorption on sand, exhibited a greater catalytic activity compared to the enzyme specimens obtained by column chromatography on CLF. Sand adsorbed GO from P. funiculosum 46.1 more effectively than aluminum oxide. It is concluded that sand may be used for fractionation of partly purified GO.