Toxicity toChrysomela tremulae (Coleoptera: Chrysomelidae) of transgenic poplars expressing a cysteine proteinase inhibitor |
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| Authors: | Jean Charles Leplé Michel Bonadé-Bottino Sylvie Augustin Gilles Pilate Véronique Dumanois Lê Tân André Delplanque Daniel Cornu Lise Jouanin |
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| Institution: | (1) Laboratorium voor Genetica and Laboratoire Associé et l'Institut National de la Recherche Agronomique (France), Universiteit Gent, B-9000 Gent, Belgium;(2) Station d'Amélioration des Arbres Forestiers, Institut National de la Recherche Agronomique, F-45160 Ardon, France;(3) Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, F-78026 Versailles Cedex, France;(4) Station de Zoologie Forestière, Institut National de la Recherche Agronomique, F-45160 Ardon, France |
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| Abstract: | The aim of this study was to test the potential of proteinase inhibitors to controlChrysomela tremulae, a beetle that causes severe damage in young plantations and in short-rotation intensive culture (SRIC) of poplar. As a first step, cysteine proteinases were determined to be the major digestive proteinases ofC. tremulae and oryzacystatin OCI, a cysteine proteinase inhibitor, was shown to inhibit this activityin vitro. The gene encoding OCI was introduced into poplar (Populus tremula ×P. tremuloides) and transgenic plants expressing OCI at a high level were selected. Feeding tests on these transgenic plants demonstrate the toxicity of OCI-producing poplar leaves againstC. tremulae larvae.J.C. Leplé and M. Bonadé-Bottino contributed equally to the research presented in this paper. |
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| Keywords: | cysteine proteinase inhibitor Coleoptera insect digestive protease transgenic poplar |
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