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CD45-positive cells are not an essential component in cardiosphere formation |
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| Authors: | Jianqin Ye Andrew J. Boyle Henry Shih Richard E. Sievers Zhi-En Wang Matthew Gormley Yerem Yeghiazarians |
| Affiliation: | 1. Department of Medicine, Division of Cardiology, University of California, San Francisco, San Francisco, CA, USA 2. Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, USA 4. Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA 5. Department of Obstetrics, Gynecology and Reproductive Biology, University of California, San Francisco, San Francisco, CA, USA 3. Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, USA 6. University of California, San Francisco, 505 Parnassus Avenue, Box 0103, San Francisco, CA, 94143-0103, USA |
| Abstract: | The cardiosphere (CS) is composed of a heterogeneous population of cells, including CD45+ cells that are bone marrow (BM)-derived. However, whether the CD45+ cells are an essential cell component in CS formation is unknown. The current study was undertaken to address this question. Cardiospheres (CSs) were harvested from 1-week post-myocardial infarction (MI) or non-MI hearts of C57BL/6 J mice. The process of CS formation was observed by timelapse photography. To analyze the role of BM-derived CD45+ cells in CS formation, CD45+ cells were depleted from populations of CS-forming cells by immunomagnetic beads. We recorded the number of CSs formed in culture from the same amount (105) of intact CS-forming cells, from CD45+-cell-depleted CS-forming cells and from CD45+ cells alone (n=6–9/cell type). CS-forming cells selectively aggregated together to form CSs by 35 h after plating. The depletion of CD45+ cells from CS-forming cells actually increased the formation of CSs (67±10 CSs/105 cells) compared with non-depleted CS-forming cells (51±6 CSs/105 cells, P<0.0001). Purified CD45+ cells from CS-forming cells did not form CSs in culture. Thus, BM-derived CD45+ cells including BM progenitors are neither necessary nor sufficient for CS formation. |
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