DNA polymorphisms of the prion doppel gene region in four different German cattle breeds and cows tested positive for bovine spongiform encephalopathy |
| |
Authors: | N Balbus A Humeny K Kashkevich I Henz C Fischer C-M Becker K Schiebel |
| |
Institution: | 1. Institut für Biochemie, Emil-Fischer-Zentrum, Universit?t Erlangen-Nürnberg, Fahrstrasse 17, 91054, Erlangen, Germany 2. Institut für Humangenetik, Universit?t Heidelberg, Im Neuenheimer Feld 366, 69120, Heidelberg, Germany
|
| |
Abstract: | Polymorphisms of the prion protein gene PRNP have been shown to influence the susceptibility/resistance to prion infections in human and sheep. In addition, the T174M
polymorphism within the flanking prion doppel gene (PRND) was thought to be involved in susceptibility to sporadic Creutzfeldt-Jacob disease. To study a possible influence of DNA
polymorphisms of the bovine PRND gene in bovine spongiform encephalopathy (BSE), previously identified and newly isolated DNA polymorphisms were genotyped
in all available German cattle that tested positive for BSE. Genotypes and calculated haplotypes were compared with breeding
bulls serving as controls. Analysis of the four major breeds Schwarzbunt (Holstein Friesian), Rotbunt (Holstein Red), Fleckvieh
(Simmental), and Braunvieh (Swiss Brown) resulted in the isolation of the previously known polymorphisms R50H and R132Q and
two novel synonymous single nucleotide polymorphisms (SNPs) C4820T and A5063T. Comparative genotype and haplotype analysis
of BSE and control animals revealed a significantly different distribution of polymorphisms C4815T and R132Q in Fleckvieh
animals but not in the other breeds tested. No association to BSE susceptibility was detectable for polymorphisms R50H and
A5063T. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|