Annual changes in the testicular activity of the river sculpin, Cottus hangiongensis Mori, with emphasis on the occurrence of aberrant spermatids during spermatogenesis

Annual changes in the spermatogenetic activity of the testis were studied histologically in the river sculpin. Coitus hangiongensis , sampled monthly from a river in southern Hokkaido, Japan. A pair of sperm reservoirs, consisting of many anastomosing lacunae, was present along the dorsomedian edge of the paired testes, and functioned also as a sperm-transporting system instead of the typical sperm duct. Spermatogenesis occurred actively in August, yielding an increasing number of mature spermatozoa in October. This process advanced, but slowly during the succeeding winter months, until March. The testis became functionally mature during the spawning period in April and May. In July, small numbers of spermatocytes were found to have appeared already, which indicated a relatively short period of post-spawning testicular regression. In November, germinal cysts containing aberrant binuclear spermatids began to appear within the seminal lobules. The paired nuclei of aberrant spermatids gradually enlarged, and the cells were released into the lumina of the seminal lobules simultaneously with the release of mature spermatozoa from the germinal cysts. During the functional maturity stage, lumina of seminal lobules which had expelled mature spermatozoa to sperm reservoirs became filled with these abnormal bodies. Discussion includes the occurrence of aberrant spermatids which resulted in the formation of 'spermatid masses' as has been described in other cottids.