Age- and Sex-dependent Differential Interaction of Nuclear <Emphasis Type="Italic">trans</Emphasis>-acting Factors with <Emphasis Type="Italic">Fmr-1</Emphasis> Promoter in Mice Brain |
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Authors: | S Prasad Kanchan Singh |
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Institution: | (1) Biochemistry & Molecular Biology Lab, Department of Zoology, Banaras Hindu University, Varanasi, 221005, UP, India |
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Abstract: | We have investigated relation between interaction of the trans-acting factors with Fmr-1 promoter and expression of FMRP isoforms in intact mouse brain as a function of age and sex. Our EMSA data reveal that among
the three complexes formed with 136 bp Fmr-1 promoter fragment, the level of complex C1 significantly increases in adult brain but decreases in old brain in comparison
to that in young. The level of total FMRP significantly decreases from young to old in the brain of both the sexes, however,
among the three isoforms, expression of the 80-kDa isoform significantly decreases in the brain of both the sexes where as
the level of 70 kDa isoform decreases in females during aging. The present finding on relation between age- and sex-dependent
interaction of trans-acting factors and expression of FMRP isoforms is novel and may be relevant for regulation of Fmr-1 gene in brain function during aging. |
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Keywords: | Aging Fmr-1 gene FMRP trans-acting factors |
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