Isolation and characterization of the exopolysaccharide produced by Daedalea quercina |
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Authors: | Matilde Manzoni Manuela Rollini |
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Affiliation: | (1) Department of Food Science and Microbiology, Industrial Microbiology Section, Università degli Studi, via Celoria 2, 20133 Milano, Italy |
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Abstract: | The production of exopolysaccharide (EPS) by a strain of the basidiomycete Daedalea quercina was investigated. Of seven different carbon sources, glucose and dextrins gave the highest crude polysaccharide yield (4.7–5 g l–1, 55–60% carbohydrate content) in shake-flask cultures, at 14 days of fermentation. Experiments carried out in a 10 l fermenter, at two different agitation speeds, gave the best results at 300 rpm, resulting in 12–14 g l–1 of crude exopolysaccharide in 9–11 days. Fractionation of the EPS samples, carried out by tangential flow ultrafiltration, evidenced a single EPS fraction (MW >30 000 Da) in samples from glucose, while two fractions (MW > 30 000 Da and 30 000 > MW > 10 000 Da) were present in samples from dextrins. Fractions characterization by HPLC and proton NMR spectroscopy revealed diversity in composition and structure in the obtained EPS: from glucose mainly an -linked mannan, and from dextrins mainly an - and -linked glucan. |
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Keywords: | Daedalea quercina exopolysaccharide mushroom submerged mycelial culture |
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