| 在高浓度1,4-丁二醇中酶促合成去B链C端六肽胰岛素 |
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| 引用本文： | 范利,崔大敷,张友尚.在高浓度1,4-丁二醇中酶促合成去B链C端六肽胰岛素[J].中国生物化学与分子生物学报,1985,1(1):33-36. |
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| 作者姓名： | 范利 崔大敷 张友尚 |
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| 作者单位： | 中国科学院上海生物化学研究所 上海
(范利，崔大敷)，中国科学院上海生物化学研究所 上海(张友尚) |
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| 摘 要： | 用含80％1,4-丁二醇的混合溶剂,以胰蛋白酶酶促,由去八肽胰岛素(DOI)合成了去六肽胰岛素(DHI),总产率为35％。1,4-丁二醇的溶解性能好,在浓度高达80—90％时不明显抑制酶活力,DOI的氨基无需保护,溶液中无高聚物或沉淀形成。
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| 关 键 词： | 酶促合成 去六肽胰岛素 1.4-丁二醇 |
| 收稿时间： | 1985-02-20 |
Enzymatic Synthesis of Deshexapeptide Insulin in the Presence of High Concentration of 1, 4-Butanediol |
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| Fan,Li Gui,DaFu Zhang,YouShang.Enzymatic Synthesis of Deshexapeptide Insulin in the Presence of High Concentration of 1, 4-Butanediol[J].Chinese Journal of Biochemistry and Molecular Biology,1985,1(1):33-36. |
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| Authors: | Fan Li Gui DaFu Zhang YouShang |
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| Institution: | Fan,Li Gui,DaFu Zhang,YouShangShanghai Institute of Biochemistry,Academia Sinica Shanghai |
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| Abstract: | 1, 4-Butanediol has been used for the cnzymatic synthesis of deshexapeptide insulin (DHI) from desoctapeptide insulin (DOT) . 1,4-Butanediol is a hydro philic solvent with good dissolving power for the reactants and will not inhibit the enzyme activity significantly even at a concentration of 80 to 90%. In addition, DOI can be used directly without protecting its amino groups. In comparison with the enzymatic synthesis of DHI in DMF/H2Oformerly reported, this method has the advantage of simplicity and higher overall yield (35%). |
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| Keywords: | Enzymatic synthesis Deshexapeptide insulin (DHI) 1 4-Butanediol |
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