Induction of DNA synthesis and callus formation from tuber tissue of Jerusalem artichoke by 2,4-dichlorophenoxyacetic acid |
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Authors: | Yasuda, Takeshi Yajima, Yasuo Yamada, Yasuyuki |
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Affiliation: | Department of Agricultural Chemistry, Kyoto University Kyoto, 606, Japan |
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Abstract: | Cellus induction was observed from Jerusalem artichoke tubertissue on a synthetic medium containing 2,4-D at 106,105 (optimum conc.) and 104 M. The first DNA synthesis(thymidine incorporation) was observed only at 2,4-D concentrationsof 105 to 104M. In 105 M 2,4-D treatedtissue, DNA synthesis increased after a 20 hr lag and reacheda maximum at 36 hr, after which it decreased. Actinomycin Dand 8-aza-guanine; inhibitors of RNA synthesis, inhibited DNAsynthesis completely. 2,4-D caused the characteristic changesin RNA and protein syntheses. In comparison with the control,RNA and protein syntheses were first repressed then inducedbefore the peak of DNA synthesis. Treatment with cycloheximide(104M) for one hour before inoculation inhibited proteinsynthesis completely for 12 hr; consequently DNA synthesis wasalso delayed. The results suggest that RNA and protein synthesesneeded for callus induction are regulated by 2,4-D in the firstDNA synthesis. (Received July 19, 1973; ) |
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