D-Gluconate is an alternative growth substrate for cultivation of Schizosaccharomyces pombe mutants |
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Authors: | Michael Hoever Brigitta Milbradt Milan Höfer |
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Institution: | (1) Botanisches Institut der Universität, Kirschallee 1, W-5300 Bonn, Federal Republic of Germany |
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Abstract: | Schizosaccharomyces pombe cells grow on d-gluconate as the sole carbon and energy source. d-Gluconate is taken up in symport with protons by a specific symporter, pH being the sole driving force. d-Gluconate uptake is independent of the sugar transporting system (e.g. for d-glucose) and of ![Delta](/content/t610811524j267w1/xxlarge916.gif) . The carrier is expressed constitutively, and its activity is not subject to glucose repression. Hence, d-gluconate is a suitable carbon and energy source for growth, when d-glucose or other hexoses have to be eliminated e.g. for selection of mutants deficient in hexose transport.Abbreviations 2-DG
2-deoxy-d-glucose
- CCCP
carbonylcyanide m-chlorophenylhydrazone
- pH
pH-gradient
- ![Delta](/content/t610811524j267w1/xxlarge916.gif)
electrical potential difference across the plasma membrane
- SD
standard deviation
- SEM
standard error of the mean
- TPP+
tetraphenylphosphonium |
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Keywords: | d-Gluconate uptake" target="_blank">d-Gluconate uptake H+-Symport Alternative growth substrate Schizosaccharomyces pombe |
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