A study on the fabrication of chemically modified bacteriorhodopsin film and its photochemical properties |
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| Authors: | Zhang Guoyan Li Baofang Zhang Jianping |
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| Institution: | Institute of Chemistry, Center for Molecular Science, Key Laboratory of Colloid and Interface Sciences, The Chinese Academy of Sciences, Beijing 100080, PR China. |
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| Abstract: | Chemically modified bacteriorhodopsin (BR) films embedded in gelatin matrix were fabricated. It was found that chemically modified BR/gelatin film remained the original conformation of BR and possessed homogeneity. Then, the photochemical conversion from all-trans O state to 9-cis P state in the chemically modified BR film/gelatin was investigated. For comparison purpose, the same conversion in the wild-type BR (BRwt) film was also presented here. To our know, it is the first report to show that the absorption changes of the P state in the chemically modified BR film were larger than in the BRwt film upon illumination with red light at the low actinic power density. |
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| Keywords: | Bacteriorhodopsin Chemical modification Conformation Homogeneity Photochemical conversion Population |
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