Biochemical and Immunological Properties of a Membrane-Bound Brain Metalloendopeptidase: Comparison with Thermolysin-Like Kidney Neutral Metalloendopeptidase

Abstract: Membrane-bound neutral metalloendopeptidase ("enkephalinase") was purified from rabbit brain and compared with a homogeneous preparation of a similar enzyme (EC 3.4.24.11) isolated from rabbit kidney. The two enzymes had the same pH optimum and the same apparent molecular weight. They showed identical specificity toward several synthetic substrates and cleaved both Met- and Leu-enkephalin at the Gly-Phe bond. Minor, but significant, differences were found between the two enzymes in the inhibitory constants determined for phosphoramidon and the N -1( R,S )-carboxy-2-phenylethyl] derivatives of phenylalanyl and alanyl- p -aminobenzoate. A guinea pig antiserum obtained against the rabbit kidney enzyme showed strong crossreactivity with the rabbit brain enzyme when tested in an anticatalytic immunoinhibition assay. Ouchterlony immunodiffusion experiments gave a pattern of precipitation consistent with partial identity of the two enzymes. The kidney enzyme, however, seemed to contain antigenic determinants not present on the brain enzyme. The data indicate that the two enzymes are identical with respect to specificity, pH optimum, and molecular weight, but show minor, although significant, differences in interaction with active-site-directed inhibitors and specific antisera.