Study on rRNA genes in Mycobacterium smegmatis |
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| Authors: | Y Suzuki T Yamada |
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| Affiliation: | Research Institute for Microbiol Diseases, Osaka University. |
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| Abstract: | The number of rRNA genes in Mycobacterium smegmatis was examined by hybridization of BamHI and SalI digests of chromosomal DNA with 3'-end-labeled 5S, 16S, 23S rRNA and tRNA. Each RNA probe gave two hybridization bands. The PstI fragments of 6.6 kilobases were cloned to pBR322. The cloned DNA was characterized by restriction endonuclease mapping, DNA-RNA hybridization, and the R-loop technique. |
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