Genomic organization of the HSET locus and the possible association of HLA-linked genes with immotile cilia syndrome (ICS) |
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Authors: | Karolina Janitz Anna Wild Stephan Beck S Savasta Giampiero Beluffi A Ziegler Armin Volz |
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Institution: | Institut für Immungenetik, Universit?tsklinikum Charité, Humboldt Universit?t zu Berlin, Spandauer Damm 130, 14050 Berlin, Germany e-mail: andreas.ziegler@charite.de, Tel.: +49-30-45053501, Fax: +49-30-45053953, DE The Sanger Centre, Wellcome Trust Genome Campus, Hinxton CB10?1SA, UK, GB Paediatric Clinic, IRCCS Policlinico S. Matteo, University of Pavia, 27100 Pavia, Italy, IT Section of Paediatric Radiology, IRCCS Policlinico S. Matteo, University of Pavia, 27100 Pavia, Italy, IT
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Abstract: | The kinesin-related protein (HSET) gene belongs to the kinesin superfamily, the members of which are involved in cellular transport processes. The HSET gene product was previously characterized by partial cDNA sequencing. The gene is located on the short arm of human Chromosome
6 (6p21.3), at the centromeric end of the major histocompatibility complex. Here, we report the genomic structure of the complete
HSET gene together with its flanking loci. Sequence analysis of the 40 kilobase (kb) cosmid clone containing the HSET gene also revealed the presence of several new genes not related to the kinesin superfamily. These include a 60S ribosomal
protein L35A-like pseudogene (rPL35A-like) on the telomeric side and a polycomb-like gene (PHF1), a copper tolerance-like gene (CUTA1) and the 5' part of the synaptic ras-GTPase-activating protein (SynGAP) gene centromeric of HSET. In addition, a complete 60S ribosomal protein L12-like (rPL12L) gene in intron 3 of the HSET gene was identified which appears to have an open reading frame. The possible involvement of the HSET gene and a β-tubulin gene (TUBB) in the pathogenesis of immotile cilia syndrome (ICS) was studied by screening two unrelated ICS families with microtubular
defects and suspected HLA linkage for mutations within the HSET gene and the TUBB gene. Four single base substitutions were detected in the HSET gene, and none in the TUBB gene. On the basis of these data, a role of the HSET and TUBB products in the pathogenesis of ICS in the two families is unlikely.
Received: 22 October / Revised: 15 February 1999 |
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Keywords: | HLA complex Immotile cilia syndrome Mutation Kinesin multigene family Human Chromosome 6 |
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