Antiproliferative effect of catechin in GRX cells |
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Authors: | Bragança de Moraes Cristina Machado Melo Denizar Alberto da Silva Santos Roberto Christ Vianna Bitencourt Shanna Mesquita Fernanda Cristina dos Santos de Oliveira Fernanda Rodriguez-Carballo Edgardo Bartrons Ramon Rosa Jose Luis Ventura Francesc Pujol Rodrigues de Oliveira Jarbas |
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Institution: | Laboratório de Biofísica Celular e Inflama??o, Pontifícia Universidade Católica do Rio Grande do Sul, PUCRS, Avenida Ipiranga 6681, prédio 12, CEP: 90619-900 Porto Alegre, Rio Grande do Sul, Brazil. |
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Abstract: | The phenolic compounds present in cocoa seeds have been studied regarding health benefits, such as antioxidant and anti-inflammatory activities. Fibrosis is a wound healing response that occurs in almost all patients with chronic liver injury. A large number of cytokines and soluble intercellular mediators are related to changes in the behavior and phenotype of the hepatic stellate cell (HSC) that develop a fibrogenic and contractile phenotype leading to the development of fibrosis. The objective of this study was to assess the catechin effect in GRX liver cells in activities such as cell growth and inflammation. The GRX cells treatment with catechin induced a significant decrease in cell growth. This mechanism does not occur by apoptosis or even by autophagy because there were no alterations in expression of caspase 3 and PARP (apoptosis), and LC3 (autophagy). The expression of p27 and p53 proteins, regulators of the cell cycle, showed increased expression, while COX-2 and IL-6 mRNA showed a significant decrease in expression. This study shows that catechin decreases cell growth in GRX cells and, probably, this decrease does not occur by apoptosis or autophagy but through an anti-inflammatory effect and cell cycle arrest. Catechin also significantly decreased the production of TGF-β by GRX cells, showing a significant antifibrotic effect. |
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