Detection of cross-links between FtsH, YidC, HflK/C suggests a linked role for these proteins in quality control upon insertion of bacterial inner membrane proteins |
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Authors: | van Bloois Edwin Dekker Henk L Fröderberg Linda Houben Edith N G Urbanus Malene L de Koster Chris G de Gier Jan-Willem Luirink Joen |
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Institution: | Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, de Boelelaan 1087, 1081 HV Amsterdam, The Netherlands. |
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Abstract: | Little is known about the quality control of proteins upon integration in the inner membrane of Escherichia coli. Here, we demonstrate that YidC and FtsH are adjacent to a nascent, truncated membrane protein using in vitro photo cross-linking. YidC plays a critical but poorly understood role in the biogenesis of E. coli inner membrane proteins (IMPs). FtsH functions as a membrane chaperone and protease. Furthermore, we show that FtsH and its modulator proteins HflK and HflC copurify with tagged YidC and, vice versa, that YidC copurifies with tagged FtsH. These results suggest that FtsH and YidC have a linked role in the quality control of IMPs. |
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Keywords: | IMP inner membrane protein TM transmembrane segment |
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