Expression vectors for the construction of hybrid Ty-VLPs |
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Authors: | Sally E. Adams S. Mark H. Richardson Susan M. Kingsman Alan J. Kingsman |
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Affiliation: | 1. Immunotherapeutics, British Biotechnology Ltd., Wattington Road, 0X4 5LY, Cowley, Oxford, UK
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Abstract: | Purification of expressed proteins can be facilitated by expressing the rccombinant protein as a fusion with a carrier protein that assembles into paniculate structures. This article describes the use of expression vectors in producing a hybrid of the yeast retrotransposon Ty, which self-assembles into virus-like particles (VLPs). Hybrid VLPs can be used in such laboratory applications as the production of polyclonal and monoclonal antibodies, structure/function analyses, the detection of important antigenic determinants, and cpitope mapping of monoclonal antibodies. |
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Keywords: | KeywordHeading" >Index Entries Expression vectors yeast retrotransposon hybrid construction purification |
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