Whole blood and urine bioactive Hepcidin-25 determination using liquid chromatography mass spectrometry |
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Authors: | Adam C Swensen Jordan G Finnell Catalina Matias Andrew J Gross John T Prince Richard K Watt John C Price |
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Institution: | 1. Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA;2. Roseman University of Health Sciences, South Jordan, UT 84095, USA |
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Abstract: | Hepcidin is a small cysteine-rich signaling peptide that regulates blood serum iron concentrations 1–4]. Patients with chronic inflammation are known to have elevated levels of hepcidin in their blood and urine and often suffer from anemia as a result 5–10]. Measuring and quantifying the amount of active hepcidin in blood and urine can help to determine the cause and severity of the anemia thereby helping physicians determine the correct course of treatment 11–16]. We have developed a simple technique to isolate, chemically modify, and concentrate hepcidin from blood and urine coupled to high-pressure liquid chromatography mass spectrometry that can accurately and reproducibly measure and quantify the active hormone. |
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Keywords: | Peptide hormone Blood Urine Anemia |
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