Synthesis by Enzymatic Catalysis VI—An Investigation of Chemical Modification of HRP by Fourier Transform Infra-Red Vibrational Spectroscopy |
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Authors: | Didier Arseguel Armand Lattes Michel Baboul ne |
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Affiliation: | Didier Arseguel ,Armand Lattes,Michel Baboulè,ne |
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Abstract: | Horseradish peroxidase was chemically conjugated on its carbohydrate moieties with short aliphatic chains (C8 and C16). An analytical method using FT.IR spectroscopy was developed to analyze this alteration in enzyme structure. This method is non-destructive, and can be applied directly to samples of the reaction mixture. More general applications of this technique are described and discussed. |
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Keywords: | Horseradish peroxidase Enzymatic modification FT.IR spectroscopy |
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