Expression of bacterial mercuric ion reductase in Saccharomyces cerevisiae. |
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| Authors: | C Rensing U Kües U Stahl D H Nies and B Friedrich |
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| Institution: | Institut für Pflanzenphysiologie und Mikrobiologie, Freie Universit?t Berlin, Germany. |
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| Abstract: | The gene merA coding for bacterial mercuric ion reductase was cloned under the control of the yeast promoter for alcohol dehydrogenase I in the yeast-Escherichia coli shuttle plasmid pADH040-2 and transformed into Saccharomyces cerevisiae AH22. The resulting transformant harbored stable copies of the merA-containing hybrid plasmid, displayed a fivefold increase in the MIC of mercuric chloride, and synthesized mercuric ion reductase activity. |
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