| 重组鼠疫菌V抗原的纯化及其豚鼠免疫保护力初探 |
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| 引用本文： | 傅林锋,戴瑞霞,应莲芳,谢榛,杨晓艳,马雅茹,万艳,薛晓红,王秉翔.重组鼠疫菌V抗原的纯化及其豚鼠免疫保护力初探[J].微生物学免疫学进展,2004,32(3):16-19. |
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| 作者姓名： | 傅林锋 戴瑞霞 应莲芳 谢榛 杨晓艳 马雅茹 万艳 薛晓红 王秉翔 |
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| 作者单位： | 1. 兰州生物制品研究所,兰州,730046
2. 青海省地方病预防控制所,西宁,811600 |
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| 摘 要： | 采用Ni^2 亲和层析方法，对用工程化大肠杆菌BL21(DE3)表达的重组鼠疫菌v抗原进行纯化，目标蛋白纯度达到90％以上。以氢氧化铝凝胶配制吸附疫苗，经二针次肌内注射免疫实验豚鼠后，对皮下注射400个致死剂量(MLD)强毒鼠疫菌攻击有一定保护效力，存活率为20％。结果表明，重组鼠疫菌V抗原有望作为改进的F1 V亚单位疫苗的主要成分。
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| 关 键 词： | 鼠疫耶尔森氏菌 重组V抗原 纯化 保护免疫应答 |
| 文章编号： | 1005-5673(2004)03-0016-04 |
| 修稿时间： | 2003年12月2日 |
Preliminary study on the purification and the immuno-protection in guinea pigs of the recombinant V antigen of yersinia pestis |
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| FU Lin-feng,DAI Rui-xia,YING Lian-fang,et al..Preliminary study on the purification and the immuno-protection in guinea pigs of the recombinant V antigen of yersinia pestis[J].Progress In Microbiology and Immunology,2004,32(3):16-19. |
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| Authors: | FU Lin-feng DAI Rui-xia YING Lian-fang |
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| Abstract: | The recombinant V antigen of Yersinia pestis expressed in Escherichia coli BL21(DE3)cells was purified by way of Ni 2+ affinity chromatography. The purity was more than 90%. An alhydrogel-adsorbed vaccine of this rV antigen has been intramuscularly injected with two doses into experimental guinea pigs. It was able to induce a effective immuno-protection against subcutaneous challenge of virulent Yersinia pestis with 400 minimal lethal doses (MLD). The survival rate was 20%. These results indicate that the rV antigen is desired to act as the major component of an improved F1+V sub-unit plague vaccine. |
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| Keywords: | Yersinia pestis Recombinant V antigen Purification Protective immune response |
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