Simple method for the analysis of tenoxicam in human plasma using high-performance liquid chromatography |
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Authors: | Jennifer L. Mason Gregory J. Hobbs |
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Affiliation: | 1. Department of Chemistry, Radiochemistry, University of Helsinki, Helsinki FI-00014, Finland;2. Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA;3. Turku PET Centre, Department of Chemistry, University of Turku, Turku FI-20520, Finland |
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Abstract: | A simple, rapid and cost-effective method for the determination of tenoxicam in human plasma is described, using ketorolac as the internal standard. The extraction procedure utilised 5% zinc sulphate and methanol. A nucleosil C18 column and 35:65 acetonitrile-water phosphate buffered mobile phase (pH 2.8) were used, with ultraviolet detection at 355 nm. The assay was linear in the range 40 ng/ml-10 μg/ml, with recovery of extraction ranging from 87 to 102%. The intra- and inter-assay reproducibility had coefficients of variation of 3.9–7.7 and 1.6% respectively. The limit of detection for this method was 40 ng/ml. |
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