Distribution of prosaposin in the rat nervous system |
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Authors: | Yoshiki Hosoda Kyojy Miyawaki Shouichiro Saito Jie Chen Xue Bing Takehiro Terashita Naoto Kobayashi Nobukazu Araki Tetsuya Shimokawa Fumihiko Hamada Akira Sano Hirotaka Tanabe Seiji Matsuda |
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Institution: | (1) Department of Neuropsychiatry, Ehime University Graduate School of Medicine, Toon, Ehime 791-0212, Japan;(2) Anatomy and Embryology, Ehime University Graduate School of Medicine, Toon, Ehime 791-0212, Japan;(3) Laboratory of Veterinary Anatomy, Faculty of Applied Biological Science, Gifu University, 1-1 Yanagida, Gifu 501-1193, Japan;(4) Medical Education, Ehime University Graduate School of Medicine, Toon, Ehime 791-0212, Japan;(5) Department of Histology and Cell Biology, School of Medicine, Kagawa University, Ikenobe, Miki, Kagawa 761-0793, Japan;(6) Department of Psychiatry, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan |
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Abstract: | Prosaposin is the precursor of four sphingolipid activator proteins (saposins A, B, C, and D) for lysosomal hydrolases and
is abundant in the nervous system and muscle. In addition to its role as a precursor of saposins in lysosomes, intact prosaposin
has neurotrophic effects in vivo or in vitro when supplied exogenously. We examined the distribution of prosaposin in the
central and peripheral nervous systems and its intracellular distribution. Using a monospecific antisaposin D antibody that
crossreacts with prosaposin but not with saposins A, B, or C, immunoblot experiments showed that both the central and peripheral
nervous systems express unprocessed prosaposin and little saposin D. Using the antisaposin D antibodies, we demonstrated that
prosaposin is abundant in almost all neurons of both the central and peripheral nervous systems, including autonomic nerves,
as well as motor and sensory nerves. Immunoelectron microscopy using double staining with antisaposin D and anticathepsin
D antibodies showed strong prosaposin immunoreactivity mainly in the lysosomal granules in the neurons in both the central
and peripheral nervous systems. The expression of prosaposin mRNA, examined using in situ hybridization, was observed in these
same neurons. Our results suggest that prosaposin is synthesized ubiquitously in neurons of both the central and peripheral
nervous systems.
Funding: This study was supported by the Ehime University INCS and in part by grants-in-aid for Scientific Research to S.M. (Exploratory
Res. 19659380) from the Japan Society for the Promotion of Science and to AS (Priority Areas 18023029) from the Ministry of
Education, Culture, Sports, Science and Technology of Japan. |
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Keywords: | Immunohistochemistry In situ hybridization Nervous system Neurotrophic factor Prosaposin Rat (Wistar) |
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