A radiation hybrid map of bovine X Chromosome (BTAX) |
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Authors: | M Elisabete J Amaral Srinivas R Kata James E Womack |
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Institution: | Departamento de Biologia, IBILCE - UNESP, S?o José Rio Preto, SP, 15054-000, Brasil, BR Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, USA, US
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Abstract: | We present a comprehensive radiation hybrid map of the bovine X chromosome (Chr) containing 20 new markers, including both
microsatellites and expressed genes. This study was conducted with a 5000-rad whole genome RH cell panel consisting of 90
hybrid cell lines. Retention frequencies of individual markers range from 7.8% for XIST to 31.1% for TGLA325. Statistical
analysis with RHMAPPER placed all the loci into five linkage groups under a LOD score criterion of 6.0. These groups could
be oriented relative to each other because they included multiple microsatellite loci from the consensus linkage map of the
X Chr. Markers included in both this RH map and the bovine cytogenetic map were in a consistent order. The comparative bovine–human
map thus generated consists of five blocks of genes, the order of which is conserved, although in the opposite direction when
presented as ideograms with p and q arms. Inversions of three blocks account for the difference in gene order across the entirety
of the two X Chrs. |
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