黄花倒水莲生长与内源激素水平动态变化关系研究北大核心CSCD

为探索黄花倒水莲春梢生理生化特性的差异以及不同内源激素的变化规律,该文对黄花倒水莲春梢的生长动态进行监测,采用间接酶联免疫吸附法(ELISA)测定脱落酸(ABA)、生长素(IAA)、赤霉素(GA)、乙烯(ETH)和玉米素核苷(ZR)五种内源激素含量的动态变化,并对两者间的相关性进行分析。结果表明:(1)黄花倒水莲春梢生长发育过程可分为快速增长期(0~12 d)、生长转折期(16~20 d)和缓慢增长期(24~32 d)三个阶段。(2)内源激素ABA、GA、ETH和ZR含量在缓慢增长期显著高于快速增长期和生长转折期,IAA含量各时期差异较小。(3)春梢长、底部叶长和叶宽在快速增长期与ABA、GA、ETH和ZR含量呈负相关,且与ZR含量具有一定显著性,与IAA含量呈正相关;生长转折期,各指标与GA、ETH和ZR含量呈正相关,与GA含量具有一定显著性,与ABA含量呈负相关;缓慢增长期,各指标与五种内源激素含量均呈正相关,与IAA和ZR含量具有一定显著性。该研究结果为生产上利用外源激素调控黄花倒水莲春梢抽出以及生长提供了理论基础。

The relationship between the growth of spring shoots and the dynamic changes of endogenous hormones in Polygala fallax

In order to explore the difference of physiological and biochemical characteristics of spring shoots and the change rule of different endogenous hormones in Polygala fallax. The growth dynamics of spring shoots were monitored, and the contents of abscisic acid(ABA), auxin(IAA), gibberellin(GA), ethylene(ETH)and zeatin nucleoside(ZR)were determined by indirect enzyme-linked immunosorbent assay(ELISA)change. The results were as follows:(1)The growth and development of the spring shoots of P. fallax could be divided into rapid growth period(0-12 d), growth transition period(16-20 d)and slow growth period(24-32 d).(2)The contents of endogenous hormones ABA, GA, ETH and ZR in slow growth period were significantly higher than those in rapid growth period and growth transition period, and the difference of IAA contents in each period was small.(3)The spring shoot length, bottom leaf length and width were negatively correlated with ABA, GA, ETH and ZR contents in the rapid growth period, and were significantly correlated with ZR content, and positively correlated with IAA content; In the growth transition period, each index was positively correlated with GA, ETH and ZR contents, and was significantly correlated with GA content, but negatively correlated with ABA content; During the slow growth period, each index was positively correlated with the contents of five endogenous hormones, and was significantly correlated with the contents of IAA and ZR. The results provide a theoretical basis for the use of exogenous hormones to regulate the spring shoot extraction and growth of P. fallax.