Study of Biochemical Pathway and Enzyme Involved in Metsulfuron-Methyl Degradation by <Emphasis Type="Italic">Ancylobacter</Emphasis> sp. XJ-412-1 Isolated from Soil |
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Authors: | Peng Lu Lei Jin Bin Liang Jing Zhang Shunpeng Li Zhaozhong Feng Xing Huang |
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Institution: | (1) Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People’s Republic of China; |
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Abstract: | Ancylobacter sp. XJ-412-1, capable of degrading metsulfuron-methyl, was isolated from sulfonylurea-contaminated soil. When metsulfuron-methyl
was provided as the sole carbon source, more than 90.5% of metsulfuron-methyl at concentration of 50 mg l−1 was degraded by strain XJ-412-1 after incubation at 30°C for 7 days. The initial degradation products of metsulfuron-methyl
(MSM), thifensulfuron-methyl (TSM), and bensulfuron-methyl (BSM) by XJ-412-1 were identified as corresponding deesterified
derivatives by liquid chromatography-mass spectrometry, which indicated a primary pathway of the deesterification of these
three sulfonylurea herbicides. The carboxyesterase activity of the cell-free extracts was assayed and strongly inhibited by
4-chloromercuribenzoic acid (PCMB), diethyl pyrocarbonate (DEPC), phenylmethylsulfonyl fluoride (PMSF), and malathion. |
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