Soil and Plant Contamination with <Emphasis Type="Italic">Mycobacterium Avium</Emphasis> subsp. <Emphasis Type="Italic">Paratuberculosis</Emphasis> After Exposure to Naturally Contaminated Mouflon Feces |
| |
Authors: | Radka Pribylova Iva Slana Marija Kaevska Jiri Lamka Vladimir Babak Jiri Jandak Ivo Pavlik |
| |
Institution: | (1) Department of Food and Feed Safety, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic;(2) Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech Republic;(3) Department of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic; |
| |
Abstract: | The aim of this study was to demonstrate the persistence of Mycobacterium avium subsp. paratuberculosis (MAP) in soil and colonization of different plant parts after deliberate exposure to mouflon feces naturally contaminated with
different amounts of MAP. Samples of aerial parts of plants, their roots, and the soil below the roots were collected after 15 weeks and examined
using IS900 real-time quantitative PCR (qPCR) and cultivation. Although the presence of viable MAP cells was not demonstrated, almost all samples were found to be positive using qPCR. MAP IS900 was not only found in the upper green parts, but also in the roots and soil samples (from 1.00 × 100 to 6.43 × 103). The level of soil and plant contamination was influenced mainly by moisture, clay content, and the depth from which the
samples were collected, rather than by the initial concentration of MAP in the feces at the beginning of the experiment. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|