Sequences in the promoter region of the chicken lysozyme gene required for steroid regulation and receptor binding |
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Authors: | R Renkawitz G Schütz D von der Ahe M Beato |
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Institution: | 1. Institute of Cell and Tumor Biology German Cancer Research Center Im Neuenheimer Feld 280 D-6900 Heidelberg, Federal Republic of Germany;2. lnstitute of Physiological Chemistry University of Marburg Deutschhausstrasse 2 D-3550 Marburg, Federal Republic of Germany |
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Abstract: | We have constructed a series of deletion mutants in the lysozyme promoter region fused to the SV40 T-antigen coding region. Regulated expression was tested after microinjection of the lysozyme deletion mutants into primary cultures of chicken oviduct cells using fluorescent antibodies against T antigen. Deletion of lysozyme gene sequences upstream of position - 164 was accompanied by loss of both progesterone- and glucocorticoid-induced expression. Using the rat liver glucocorticoid receptor for binding studies, two separate binding sites have been identified: a strong binding site that is destroyed by deletion of lysozyme sequences between positions -74 and -39 and a weaker binding site contained between positions -208 and -161 upstream of the lysozyme cap site. |
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