Replication of double-stranded RNA in mycovirus from the plant pathogenic fungus, Fusarium solani |
| |
Authors: | Masahiro Nogawa Aiko Nakatani Keiichi Gonda Makoto Shimosaka mitsuo Okazaki |
| |
Institution: | Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Veda, Nagano 386, Japan |
| |
Abstract: | Abstract A mycovirus (named FusoV) from the plant pathogenic fungus Fusarium solani possessed two types of double-stranded (ds) RNA genome, designated Ml and M2. RNA-dependent RNA polymerase activity was detected in FusoV particle fractions. An in vitro RNA polymerase reaction using purified FusoV particles that was supplemented with NTPs revealed the synthesis of single-stranded (ss) RNA species and a subsequent formation of dsRNAs having the same size as Ml and M2. The ssRNA species synthesized in the first stage were proved to be of positive polarity (coding strand) for both M1 and M2 by dot blot hybridization analysis. These results suggest that FusoV genomic dsRNA replicates in a conservative manner. |
| |
Keywords: | Fusarium solani Double-stranded RNA In vitro replication RNA-dependent RNA polymerase |
本文献已被 ScienceDirect 等数据库收录! |