EST–SSR markers for asparagus genetic diversity evaluation and cultivar identification |
| |
Authors: | Marco Caruso Claire T Federici Mikeal L Roose |
| |
Institution: | (1) Dipartimento di Ortofloroarboricoltura e Tecnologie Agroalimentari, Sezione Arboricoltura, University of Catania, Catania, 95123, Italy;(2) Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA |
| |
Abstract: | Simple sequence repeat (SSR) markers generated from expressed sequence tag (EST) sequences represent useful tools for genotyping
and their development is relatively easy because of the public availability of EST databases. We report design and application
of EST–SSRs to assess the level of genetic diversity among thirty-five asparagus cultivars and to fingerprint DePaoli, a new
variety released by University of California, Riverside. DNA was isolated from bulks of pooled cladophylls coming from five
plants of each variety to reduce the number of DNA extractions and PCR reactions. Allele frequencies were estimated from the
intensity of the bands in two bulks and two individual plant samples for each variety. Although asparagus varieties derive
from a limited germplasm pool, eight EST–SSR loci differentiated all of the analyzed cultivars. Moreover, UPGMA (unweighted
pair group method with arithmetic mean) and neighbor-joining trees, as well as principal components analysis separated the
cultivars into clusters corresponding to the geographical areas where they originated. |
| |
Keywords: | DNA markers Microsatellite |
本文献已被 SpringerLink 等数据库收录! |
|