Effects of exogenous abscisic acid (ABA) on cucumber seedling leaf carbohydrate metabolism under low temperature |
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Authors: | Fan-Zhen Meng Li-Ping Hu Shao-Hui Wang Xiao-Lei Sui Li Wei Yu-Xia Wei Jian-Lei Sun Zhen-Xian Zhang |
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Institution: | (1) College of Agronomy and Biotechnology, China Agriculture University, Beijing, 100094, China;(2) Plant Science and Technology Department, Beijing University of Agriculture, Beijing, 102206, China;(3) College Agronomy of Guizhou University, Guiyang, 550025, China |
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Abstract: | Seedlings with four true leaves of cucumbers (Cucumis sativus L.), Guonong No.25 (a cold-tolerant cultivar) and Guonong No.41 (a cold sensitive cultivar), were grown under normal or low
temperature conditions: 25°C/18°C or 15°C/8°C (day/night). The seedlings of Guonong No.25 under low temperature were also
treated with or without exogenous ABA. The purpose of our study was to find out the effects of low temperature and exogenous
ABA application on the carbohydrate metabolism in the cucumber plants. Time course changes of carbohydrate contents and activities
of stachyose synthase and alkaline α-galactosidase in the seedling leaves were investigated after the treatment. Our results
show that compared to the seedlings under temperatures of 25°C/18°C, the seedlings of the both tested genotypes under 15°C/8°C
(day/night) have significantly higher contents of all measured soluble carbohydrates. Significant difference in stachyose
synthase activity is observed between the two genotypes under normal temperature or low temperature. Under normal temperature,
leaf stachyose synthase activity in Guonong No.41 is higher than that in Guonong No.25. The stachyose synthase activity of
Guonong No.41 decreases sharply under low temperature, but that of Guonong No.25 increases 3 days after treatment and then
decreases to the original level. In contrast, there is no significant genotypic difference in alkaline α-galactosidase activity.
Additionally, compared to the control seedlings treated with 0 μM ABA, the seedlings treated with 50 and 150 μM ABA accumulate
substantial amounts of all tested soluble carbohydrates except galactose whereas 250 μM ABA treated seedlings show decreased
levels of all these soluble carbohydrates. Stachyose synthase activity increases significantly upon 50 and 150 μM ABA treatments.
Fan-zhen Menga, Li-ping Hu, and Shao-hui Wang contributed equally to the paper. |
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Keywords: | Carbohydrates Cucumber seedling Exogenous abscisic acid Low temperature Stachyose synthase |
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