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Nucleotide sequences and stability of a Nicotiana nuclear DNA segment possessing autonomously replicating ability in yeast |
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| Authors: | Takeshi Ohtani Shigeot Kiyokawa Toshifumi Ohgawara Hiroshi Harada Hirofumi Uchimiya |
| Affiliation: | (1) Institute of Biological Sciences, University of Tsukuba, Sakura-mura, 305 Ibaraki, Japan;(2) Present address: Kirin Brewery Co., LTD., Plant Research Center, Kitsuregawa, Shioya-gun, 329-14 Tochigi, Japan;(3) Present address: Bioscience Research Laboratory, Kikkoman Corp., Nodacity, Japan |
| Abstract: | The nucleotide sequence of a tobacco (Nicotiana tabacum) chromosomal DNA segment(t3-ars) capable of replication in yeast (ars: autonomously replicating sequences) is presented. The subcloned region (618 bp) contained 11 bp consensus (5 A/TTTTATPuTTTA/T 3) essential for several yeast ars, and 73% A and T. Unique 70 bp repetitive sequences resided next to this sequence. Thirty-two bp AT repeats were also seen in the neighbourhood of the repetitive sequence. The hybrid plasmid containing t3-ars was mitotically stabilized by the help of yeast centromere (CEN4). |
| Keywords: | tobacco nuclear DNA autonomous replication |
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