Anionic phospholipids can mediate membrane insertion of the anionic part of a bound peptide |
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| Authors: | J. M. Leenhouts P. W. J. van den Wijngaard A. I. P. M. de Kroon B. de Kruijff |
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| Affiliation: | Department of Biochemistry of Membranes (CBLE), Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands |
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| Abstract: | The effect of anionic lipids on the membrane insertion of a carboxyl group on a specially designed palmitoylated peptide was studied, using tryptophan fluorescence. It is demonstrated that the negatively charged membrane surface of mixed phosphatidylcholine/phosphatidylglycerol small unilamellar vesicles enhances the protonation of the C-terminal carboxyl group, and the subsequent insertion of that part of the peptide. |
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| Keywords: | Anionic phospholipid Peptide insertion Fluorescence spectroscopy Surface pH |
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