Cell/adsorbent interactions in expanded bed adsorption of proteins |
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Authors: | Jan Feuser Joachim Walter Maria-Regina Kula Jörg Thömmes |
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Institution: | (1) Boehringer Ingelheim Pharma KG, Birkendorfer Straße 65, 88397 Biberach an der Riss, Germany;(2) Institut für Enzymtechnologie, Heinrich-Heine Universität Düsseldorf, Stetternicher Forst, 52426 Jülich, Germany |
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Abstract: | Expanded bed adsorption (EBA) is an integrated technology for the primary recovery of proteins from unclarified feedstock. A method is presented which allows a qualitative and quantitative understanding of the main mechanisms governing the interaction of biomass with fluidised resins. A pulse response technique was used to determine the adsorption of various cell types (yeast, Gram positive and Gram negative bacteria, mammalian cells and yeast homogenate) to a range of commercially available matrices for EBA. Cells and cell debris were found to interact with the ligands of agarose based resins mainly by electrostatic forces. From the adsorbents investigated the anion exchange matrix showed the most severe interactions, while cation exchange and affinity adsorbents appeared to be less affected. Within the range of biologic systems under study E. coli cells had the lowest tendency of binding to all matrices while hybridoma cells attached to all the adsorbents except the protein A affinity matrix. The method presented may be employed for screening of suitable biomass/adsorbent combinations, which yield a robust and reliable initial capture step by expanded bed adsorption from unclarified feedstock. |
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Keywords: | expanded bed adsorption fluidisation initial recovery protein purification |
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