DR antigen expression on ovarian carcinoma cells does not correlate with their capacity to elicit an autologous proliferative response |
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Authors: | Maria Di Bello Valeria Lucchini Stefania Chiari Roberto Colleoni Nicoletta Colombo Alberto Mantovani Paola Allavena |
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Institution: | (1) Laboratory of Human Immunology, Istituto di Ricerche Farmacologiche Mario Negri, Via Eritrea, 62, I-20157 Milan, Italy;(2) Department of Histopathology, S. Gerardo Hospital, Monza, Milan, Italy;(3) Department of Obstetric and Gynecology, S. Gerardo Hospital, Monza, Milan, Italy |
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Abstract: | Summary Expression of HLA-DR antigens by purified preparations of human ovarian carcinoma cells freshly isolated from surgical specimens was examined in parallel with the capacity of tumor cells to elicit a blastogenic response from autologous lymphocytes in mixed lymphocyte-tumor culture (MLTC) assay. Of 21 tumor preparations, 11 (52%) reacted with monoclonal antibodies 279 and/or 949 specific for a monomorphic determinant of HLA-DR antigens, with heterogeneous positivity, ranging between 30% and 95%. In this series of patients positive MLTC occurred in 8/21 individual experiments. The HLA-DR expression was proportionally similar in tumors giving positive MLTC (4/8=50%) and negative MLTC (7/13=53%). The lack of correlation between DR expression on tumor cells and stimulatory activity in autologous MLTC and the fact that DR-negative tumors could induce lymphocyte stimulation, support the hypothesis that blastogenesis occurs upon recognition of tumor-associated antigens, different from DR molecules, possibly tumor-specific antigens. |
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