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A convenient protocol for extraction and purification of DNA from Fragaria
Authors:José A Mercado  Iman El Mansouri  Silvia Jiménez-Bermúdez  Fernando Pliego-Alfaro  Miguel A Quesada
Institution:(1) Departamento Biología Vegetal, Universidad de Málaga, Campus de Teatinos, 29071 Málaga, Spain
Abstract:Summary In this paper we describe a simple and efficient DNA extraction protocol for Fragaria species, a highly recalcitrant genus due to the large amount of polyphenols and polymeric carbohydrates present in strawberry tissues. The protocol yields a high quality DNA that can be amplified by polymerase chain reaction and digested with restriction endonucleases.
Keywords:DNA            Fragaria            strawberry
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