A pathway for biodegradation of 1-naphthoic acid by Pseudomonas maltophilia CSV89 |
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Authors: | Prashant S Phale Milind C Mahajan Chelakard S Vaidyanathan |
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Institution: | (1) Department of Biochemistry, Indian Institute of Science, 560 012 Bangalore, India;(2) Present address: Department of Genetics, Boyer Center for Molecular Medicine, Yale University School of Medicine, 06536-0812 New Haven, CT, USA |
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Abstract: | Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mass spectrometry. The involvement of various metabolites as intermediates in the pathway was established by demonstrating relevant enzyme activities in cell-free extracts, oxygen uptake and transformation of metabolites by the whole cells. The results obtained from such studies suggest that the degradation of 1-naphthoic acid is initiated by double hydroxylation of the aromatic ring adjacent to the one bearing the carboxyl group, resulting in the formation of 1,2-dihydroxy-8-carboxynaphthalene. The resultant diol was oxidized via 3-formyl salicylate, 2-hydroxyisophthalate, salicylate and catechol to TCA cycle intermediates. |
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Keywords: | Pseudomonas 1-Naphthoic acid Hydrocarbon degradation Hydroxylation Ring cleavage |
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